Separation and determination of sugars by reversed-phase high-performance liquid chromatography after pre-column microwave-assisted derivatization |
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Authors: | Fariborz Momenbeik Jafar H Khorasani |
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Institution: | (1) Department of Chemistry, University of Isfahan, 81746-73441 Isfahan, Iran |
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Abstract: | In this study the possibility of derivatizing sugars using microwave irradiation was investigated. The amount of reagent,
irradiation intensity, and derivatization time were optimized. In the derivatization of sugars with p-nitroaniline the reaction is complete within 5 min at 600 W when the p-nitroaniline-to-sugar and NaBH3CN-to-sugar mole ratios were above 1.4 and 3.1, respectively. A Doehlert design was used to optimize the mobile phase for
separation of p-nitroaniline-labeled sugars; and the best separation was obtained by use of 0.01 mol L−1 acetate buffer at pH 4.40 containing 11.0% acetonitrile. Analysis using this method was highly sensitive and analysis time
was short. Finally, a food sample was analyzed using the proposed method. |
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Keywords: | Sugars Microwave-assisted reaction High-performance liquid chromatography p-Nitroaniline Doehlert design |
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