Enantioselective gas chromatographic analysis of aqueous samples by on-line derivatisation. Application to enzymatic reactions |
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Authors: | Mommers John H M de Wildeman Stefaan M A Koolen Wilma A F Duchateau Alexander L L |
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Affiliation: | DSM Resolve, 6160 MD Geleen, The Netherlands. John.Mommers@dsm.com |
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Abstract: | A new gas chromatography (GC) method is presented for analysing both the conversion and the enantiomeric excess (e.e.) of samples from alcohol dehydrogenase reactions. The chiral compounds studied were a series of saturated, straight chain alcohols, ranging from 2-butanol to 2-heptanol. The alcohols were converted to the corresponding trifluoroacetylated derivatives by injecting trifluoroacetic anhydride onto the column shortly after injection of the aqueous samples in split-injection mode (1:100) onto a Chiraldex G-TA capillary GC column. Injecting seven hundred aqueous enzymatic reaction mixtures according to the above-mentioned procedure revealed no noticeable loss of column performance. Using the new GC method, conventional sample work-up procedures such as extraction and off-line derivatisation are eliminated and throughput of samples is significantly enhanced. |
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