Backfilling‐Free Strategy for Biopatterning on Intrinsically Dual‐Functionalized Poly[2‐Aminoethyl Methacrylate‐co‐Oligo(Ethylene Glycol) Methacrylate] Films |
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| Authors: | Dr Bong Soo Lee Dr Juno Lee Gyeongyeop Han EunRae Ha Prof Dr Insung S Choi Prof Dr Jungkyu K Lee |
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| Institution: | 1. Department of Chemistry and Center for Cell-Encapsulation Research, KAIST, Daejeon, South Korea;2. Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu, South Korea |
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| Abstract: | We demonstrated protein and cellular patterning with a soft lithography technique using poly2‐aminoethyl methacrylate‐co‐oligo(ethylene glycol) methacrylate] films on gold surfaces without employing a backfilling process. The backfilling process plays an important role in successfully generating biopatterns; however, it has potential disadvantages in several interesting research and technical applications. To overcome the issue, a copolymer system having highly reactive functional groups and bioinert properties was introduced through a surface‐initiated controlled radical polymerization with 2‐aminoethyl methacrylate hydrochloride (AMA) and oligo(ethylene glycol) methacrylate (OEGMA). The prepared poly(AMA‐co‐OEGMA) film was fully characterized, and among the films having different thicknesses, the 35 nm‐thick biotinylated, poly(AMA‐co‐OEGMA) film exhibited an optimum performance, such as the lowest nonspecific adsorption and the highest specific binding capability toward proteins. |
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| Keywords: | non-biofouling synthetic polymers protein and cellular pattern surface organic chemistry surface-functionalizable biomaterials surface-initiated controlled radical polymerization |
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