DNA Triplexes‐Guided Assembly of G‐Quadruplexes for Constructing Label‐free Fluorescent Logic Gates |
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| Authors: | Lijun Xu Shanni Hong Xiaoqiang Shen Lu Zhou Jine Wang Jianye Zhang Prof. Dr. Renjun Pei |
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| Affiliation: | 1. Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou, China;2. University of Chinese Academy of Sciences, Beijing, China;3. School of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China |
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| Abstract: | Assembly of G‐quadruplexes guided by DNA triplexes in a controlled manner is achieved for the first time. The folding of triplex sequences in acidic conditions brings two separated guanine‐rich sequences together and subsequently a G‐quadruplex structure is formed in the presence of K+. Based on this novel platform, label‐free fluorescent logic gates, such as AND, INHIBIT, and NOR, are constructed with ions as input and the fluorescence of a G‐quadruplex‐specific fluorescent probe NMM as output. |
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| Keywords: | DNA fluorescent probes G-quadruplexes self-assembly triplex DNA |
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