| 烟酸生物转化产物的双波长检测研究 |
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| 引用本文： | 尹祖建,罗晖,常雁红,苏厚波,肖宝清.烟酸生物转化产物的双波长检测研究[J].化学分析计量,2010(3):55-57. |
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| 作者姓名： | 尹祖建 罗晖 常雁红 苏厚波 肖宝清 |
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| 作者单位： | 1. 北京科技大学环境工程系,北京100083;
2. 北京科技大学生物科学与技术系,北京100083; |
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| 基金项目： | 国家重点基础发展计划（2007CB714304） |
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| 摘 要： | 建立了一种利用双波长分光光度法测定烟酸酶法转化液中烟酸和6-羟基烟酸浓度的方法,选择的波长为266.5、300 nm。对烟酸生物转化产物的混合物进行分析,烟酸测定结果的标准标准偏差不大于1.30%(n=6),6-羟基烟酸测定结果的相对标准标准偏差不大于1.54%(n=6)。烟酸和6-羟基烟酸的加标回收率为95.1%～105.1%,检出限分别为0.9 mg/L和0.26 mg/L。该法与常规的HPLC方法相比,快速、准确、适于大量样品的测定。
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| 关 键 词： | 双波长分光光度法 烟酸 6-羟基烟酸 |
DETERMINATION OF NICOTINIC ACID BIOTRANSFORMED MIXTURE BY DUAL WAVELENGTH ULTRAVIOLENT SPECTROPHOTOMETRY |
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| Yin Zujian,Luo Hui,Chang Yanhong,Su Houbo,Xiao Baoqing.DETERMINATION OF NICOTINIC ACID BIOTRANSFORMED MIXTURE BY DUAL WAVELENGTH ULTRAVIOLENT SPECTROPHOTOMETRY[J].Chemical Analysis And Meterage,2010(3):55-57. |
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| Authors: | Yin Zujian Luo Hui Chang Yanhong Su Houbo Xiao Baoqing |
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| Institution: | 1. Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China; 2. Department of Biological Science and Technology, University of Science and Technology Beijing, Beijing 100083, China) |
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| Abstract: | A method for determination of nicotinic acid and 6-hydroxynicotinic acid in enzymatic transformed mixture by double - wavelength UV spectrophotometry was developed, and the wavelength of 266.5 nm and 300 nm were selected. The relative standard deviation for nicotinic acid was less than 1.3% and 6-hydroxynicotinic acid was less than 1.54%. The recovery of nicotinic acid and 6-hydroxynicotinic acid was 95.1% - 105. I%. The detection limits for nicotinc and 6-hydroxynicotinic were 0.9 mg/L and 0.26 mg/L, respectively. The dual wavelength spectrophotometry is rapid, accurate and superior to conventional HPLC method, especially in bulk samples determination. |
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| Keywords: | dual wavelength spectrophotometry nicotinic acid 6-hydroxynicotinic acid |
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