| 甲壳素纯度的高效液相色谱法测定 |
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| 引用本文： | 朱晓兰,夏文水. 甲壳素纯度的高效液相色谱法测定[J]. 分析测试学报, 2003, 22(4): 9-13 |
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| 作者姓名： | 朱晓兰 夏文水 |
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| 作者单位： | 1. 中国科技大学,烟草研究中心,安徽,合肥,230052
2. 无锡轻工业大学,食品学院,江苏,无锡,214036 |
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| 摘 要： | 由于生物材料中甲壳素或壳聚糖是大分子，不溶于水，以及与蛋白质等结合在一起而难以定量测定，工业生产中尚无甲壳素或壳聚糖纯度的标准测定方法；采用甲壳素水解生成氨基葡萄糖，再用氯甲酸芴甲酯(FMOC—C1)进行衍生，利用液相色谱技术对衍生产物进行分析；结果表明，HPLC法是生物材料中甲壳素的一种灵敏的、特有的定性定量分析方法，为生物材料及工业化生产中甲壳素纯度的测定提供了一个方便的手段。
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| 关 键 词： | 甲壳素 纯度 高效液相色谱法 测定 衍生化 氨基葡萄糖盐酸盐 |
| 文章编号： | 1004-4957(2003)04-0009-04 |
Determination of Purity of Chitin by High Performance Liquid Chromatography |
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| ZHUXiao_lan ,XIA Wen_shui. Determination of Purity of Chitin by High Performance Liquid Chromatography[J]. Journal of Instrumental Analysis, 2003, 22(4): 9-13 |
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| Authors: | ZHUXiao_lan XIA Wen_shui |
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| Affiliation: | ZHUXiao_lan 1,XIA Wen_shui 2 |
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| Abstract: | Chitin is a kind of biological material with high relative molecular mass.Because of its unsolubility in water and combination with protein,it is difficult to determine its purity.A method for the determination of chitin purity is presented by high performance liquid chromatography.Chitin was hydrolyzed to glucosamine and then the products was derivatized using9_fluorenylmethoxycarbonyl chloride as a derivatization reagent.The results showed that the HPLC method offered a sensitive and specific tool for the quantification of chitin in biological material and industrial production. |
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| Keywords: | Chitin High performance liquid chromatography Derivatization Glucosamine |
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