| 固定Fe3＋的磁性微球分离富集鼠肝中铁结合蛋白的初步研究 |
| |
| 引用本文： | 方彩云,王胜,刘清萍,晏国金,陆豪杰,杨芃原.固定Fe3＋的磁性微球分离富集鼠肝中铁结合蛋白的初步研究[J].化学学报,2008,66(19). |
| |
| 作者姓名： | 方彩云 王胜 刘清萍 晏国金 陆豪杰 杨芃原 |
| |
| 作者单位： | 1. 复旦大学化学系,上海,200433
2. 复旦大学化学系,上海,200433;复旦大学生物医学研究院,上海,200032;复旦大学教育部创新科学仪器工程中心,上海,200433 |
| |
| 基金项目： | 上海市重点学科建设项目，国家高技术研究发展计划(863计划)探索课题 |
| |
| 摘 要： | 采用键合Fe3 的纳米材料分离富集了大鼠肝脏中的铁结合蛋白质组,并进行了质谱分析.在相同的起始富集蛋白质量以及相同的吸附和洗脱条件下,键合了Fe3 的磁性纳米材料比未键合金属离子的空白材料富集了更多的蛋白质,经质谱鉴定得到42个蛋白质,主要包括代谢酶类、呼吸链主要成员、氧化还原蛋白、转运蛋白、血红蛋白等.
|
| 关 键 词： | 磁性纳米材料 铁结合 蛋白质组 |
Preliminary Study of Iron-binding Proteins in Rat Liver by Magnetic Microsphere Immobilized Iron Affinity Chromatography |
| |
| FANG,Cai-Yun,WANG,Sheng,LIU,Qing-Ping,YAN,Guo-Quan,LU,Hao-Jie,YANG,Peng-Yuan.Preliminary Study of Iron-binding Proteins in Rat Liver by Magnetic Microsphere Immobilized Iron Affinity Chromatography[J].Acta Chimica Sinica,2008,66(19). |
| |
| Authors: | FANG Cai-Yun WANG Sheng LIU Qing-Ping YAN Guo-Quan LU Hao-Jie YANG Peng-Yuan |
| |
| Abstract: | Iron-binding proteome of rat liver were enriched by Fe3 -immobilized silica nanomaterials and analyzed by HPLC-MS. More proteins were identified with enrichment of the Fe3 -immobilized silica nanomaterials than those with normal silica nanomaterials when the starting proteins with the same amount and the same conditions of absorption and elution were used. Finally, 42 iron-binding proteins were identified, which were mainly referred to enzymes of metabolism, members of respiratory chain, proteins involved in oxidation-reduction, transporters and hemoglobins. |
| |
| Keywords: | magnetic nanomaterial iron-binding proteome |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
