Nitric oxide and singlet oxygen photo-generation by light irradiation in the phototherapeutic window of a nitrosyl ruthenium conjugated with a phthalocyanine rare earth complex |
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Authors: | Simone Aparecida Cicillini Ana Carolina Lemos Prazias Antonio Claudio Tedesco Osvaldo Antonio Serra Roberto Santana da Silva |
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Affiliation: | aFaculdade de Ciências Farmacêuticas de Ribeirão Preto, USP, Av do Café s/n, Ribeirão Preto-SP 14040-903, Brazil;bDepartamento de Química da FFCLRP-USP, Av Bandeirantes 3900, Ribeirão Preto-SP 14040-901, Brazil |
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Abstract: | The photochemical, photophysical and photobiological studies of a mixture containing cis-[Ru(H-dcbpy−)2(Cl)(NO)] (H2-dcbpy = 4,4′-dicarboxy-2,2′-bipyridine) and Na4[Tb(TsPc)(acac)] (TsPc = tetrasulfonated phthalocyanines; acac = acetylacetone), a system capable of improving photodynamic therapy (PDT), were accomplished. cis-[Ru(H-dcbpy−)2(Cl)(NO)] was obtained from cis-[Ru(H2-dcbpy)2Cl2]·2H2O, whereas Na4[Tb(TsPc)(acac)] was obtained by reacting phthalocyanine with terbium acetylacetonate. The UV–Vis spectrum of cis-[Ru(H-dcbpy−)2(Cl)(NO)] displays a band in the region of 305 nm (λmax in 0.1 mol L−1 HCl)(π–π*) and a shoulder at 323 nm (MLCT), while the UV–Vis spectrum of Na4[Tb(TsPc)(acac)] presents the typical phthalocyanine bands at 342 nm (Soret λmax in H2O) and 642, 682 (Q bands). The cis-[Ru(H-dcbpy−)2(Cl)(NO)] FTIR spectrum displays a band at 1932 cm−1 (Ru–NO+). The cyclic voltammogram of the cis-[Ru(H-dcbpy−)2(Cl)(NO)] complex in aqueous solution presented peaks at E = 0.10 V (NO+/0) and E = −0.50 V (NO0/−) versus Ag/AgCl. The NO concentration and 1O2 quantum yield for light irradiation in the λ > 550 nm region were measured as [NO] = 1.21 ± 0.14 μmol L−1 and øOS = 0.41, respectively. The amount of released NO seems to be dependent on oxygen concentration, once the NO concentration measured in aerated condition was 1.51 ± 0.11 μmol L−1 The photochemical pathway of the cis-[Ru(H-dcbpy−)2(Cl)(NO)]/Na4[Tb(TsPc)(acac)] mixture could be attributed to a photoinduced electron transfer process. The cytotoxic assays of cis-[Ru(H-dcbpy-)2(Cl)(NO)] and of the mixture carried out with B16F10 cells show a decrease in cell viability to 80% in the dark and to 20% under light irradiation. Our results document that the simultaneous production of NO and 1O2 could improve PDT and be useful in cancer treatment. |
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Keywords: | Nitric oxide Photoinduced electron transfer Phthalocyanine Rare earth and ruthenium |
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