A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography |
| |
| Authors: | de Sousa João P B Bueno Paula C P Gregório Luiz E da Silva Filho Ademar A Furtado Niege A J C de Sousa Márcia L Bastos Jairo K |
| |
| Institution: | 1. Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto da Universidade de S?o Paulo, Ribeir?o Preto, SP, Brazil. Fax: +55‐16‐3602‐4879;2. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto da Universidade de S?o Paulo, Ribeir?o Preto, SP, Brazil;3. Núcleo de Pesquisa em Ciências Exatas e Tecnológicas da Universidade de Franca, Franca, SP, Brazil;4. Departamento de Vigilancia Sanitária de Goiania, Goiania, Brazil |
| |
| Abstract: | A sensitive and reliable RP-HPLC method was developed using a C18 CLC-ODS (M) - 4.6x250 mm(2)column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2-dimethyl-6-carboxyethenyl-2H-1-benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83-533.33 microg/mL. |
| |
| Keywords: | Apis mellifera Brazilian green propolis HPLC Phenolic compounds |
| 本文献已被 PubMed 等数据库收录！ |
|
