Determination of 48 pesticides and their main metabolites in water samples by employing sonication and liquid chromatography-tandem mass spectrometry |
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Authors: | Fenoll José Hellín Pilar Martínez Carmen M Flores Pilar Navarro Simón |
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Affiliation: | a Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario, IMIDA, C/Mayor s/n, La Alberca, 30150 Murcia, Spain b Departamento de Química Agrícola, Geología y Edafología. Facultad de Química. Universidad de Murcia. Campus de Espinardo, 30100 Murcia, Spain |
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Abstract: | In this work, a rapid and sensitive analytical multiresidue method has been developed for the simultaneous determination of 48 pesticides and 19 metabolites in waters (tap, leaching and sewage), using liquid chromatography-tandem mass spectrometry (LC-MS/MS) with triple quadrupole in selected reaction monitoring (SRM) mode. The procedure involves initial single phase extraction of samples with acetonitrile by sonication, followed by liquid-liquid partition aided by “salting out” process using NaCl. Matrix influence on recoveries was evaluated for the three waters. More than 50% of the compound presented very low signal suppression. The method presents good linearity over the range assayed 10-500 μg L−1 and the most frequent detection limits was 0.05 ng mL−1. The average recovery by the LC-MS/MS method obtained for these compounds varied from 74.6 to 111.2% with a relative standard deviation between 2.5 and 8.9%. The proposed method was used to determine pesticides levels in leaching water samples from 5 lysimeters from an experimental greenhouse located in Murcia. |
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Keywords: | Multiresidue Waters Metabolites Pesticides Liquid chromatography |
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