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Constant-wavelength synchronous spectrofluorimetry for determination of riboflavin in anchovies |
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| Authors: | T J López y López-Leytón M A Lage Yusty and M E Alvarez Pi?eiro |
| Institution: | (1) Instituto de Investigación y Análisis Alimentarios, Laboratorio de Bromatología, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus, s/n, E-15706-Santiago de Compostela, Spain, ES;(2) Dpto de Q. Analítica, Nutrición y Bromatología, Area Nutrición y Bromatología, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus, s/n, E-15706-Santiago de Compostela, Spain, ES |
| Abstract: | Constant-wavelength synchronous spectrofluorimetry was used for the analysis of riboflavin in anchovies. Synchronous fluorescence spectra were recorded between 300 and 600 nm at a scan rate of 240 nm min–1 and with excitation and emission slit-widths both set to 5 nm. The excitation-emission wavelength difference was 65 nm. The fluorescence was measured by peak-area base between 430–509 nm. Recovery was higher than 90.8%. Received: 22 December 1997 / Revised: 25 February 1998 / Accepted: 25 March 1998 |
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