The preparation of sorbents for the analysis of human antithrombin III by means of high performance affinity chromatography |
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| Authors: | A L Dawidowicz T Rauckyte J Rogalski |
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| Institution: | (1) Department of Chemical Physics and Physicochemical Separation Methods, Maria Curie Sklodowska University, M.C. Sklodowska Square 3, 20-031 Lublin, Poland;(2) Department of Biochemistry, Maria Curie Sklodowska University, M.C. Sklodowska Square 3, 20-031 Lublin, Poland |
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| Abstract: | Summary Antithrombin III (AT III) is an anticoagulant present in blood. It is responsible among other things for blood coagulation and the wrong amount can lead to various health problems. The level of AT III can be taken as an indicator of many pathological states. Due to the very complex composition of blood, high performance affinity chromatography seems to be one of the best methods for the quantitative determination of AT III. The present paper deals with the preparation and properties of sorbents for AT III analysis. The behaviour of the chromatographic packings obtained by the bonding of heparin (used as a complementary ligand interacting with AT III) to cross-linked polysaccharide layers deposited on controlled porosity glass is discussed. |
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| Keywords: | Affinity chromatography Controlled porosity glass Antithrombin III Heparin immobilization Serum analysis |
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