Targeted and ultrasound-triggered cancer cell injury using perfluorocarbon emulsion-loaded liposomes endowed with cancer cell-targeting and fusogenic capabilities |
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| Institution: | 1. Institute for Frontier Science Initiative, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;2. Faculty of Natural System, Institute of Science and Engineering, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;3. Institute of Nature and Environmental Technology, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan;1. Department of Mechanical Engineering and Center for Nanoscience and Nanobiotechnology, Boston University, Boston, Massachusetts, USA;2. Department of Biomedical Engineering, Boston University, Boston, Massachusetts, USA;1. Institute of Biomedical Engineering, Old Road Campus Research Building, University of Oxford, Oxford OX3 7DQ, UK;2. Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, Shandong University, Jinan 250100, China;3. School of Chemistry, Clayton Campus, Monash University Victoria, 3800, Australia;4. Mechatronics and Bioengineering Science Research Groups, Faculty of Engineering and the Environment, University of Southampton, Southampton, UK;1. Institut Galien Paris-Sud, CNRS, Univ. Paris-Sud, Université Paris-Saclay, 92296 Châtenay-Malabry, France;1. LAQV/Requimte, Department of Chemistry, Faculty of Science and Technology, NOVA University of Lisbon, Campus de Caparica, 2829-516, Caparica, Portugal;2. Univ Coimbra, CIEPQPF, Department of Chemical Engineering, Rua Sílvio Lima, Polo II-Pinhal de Marrocos, 3030-790, Coimbra, Portugal |
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| Abstract: | This study investigated the targeting and ultrasound-triggered injury of cancer cells using anticancer drug-free liposomes that contained an emulsion of perfluoropentane (ePFC5) and were co-modified with avidin as a targeting ligand for cancer cells and the hemagglutinating virus of Japan (HVJ) envelope to promote liposome fusion with the cells. These liposomes are designated as ePFC5-loaded avidin/HVJ liposomes. ePFC5-loaded liposomes were sensitized to ultrasound irradiation. Liposomes modified with avidin alone (avidin liposomes) showed binding to MCF-7 human breast cancer cells, and liposomes modified with HVJ envelope alone (HVJ liposomes) were found to fuse with MCF-7 cells. The irradiation of MCF-7 cells with 1 MHz ultrasound (30 s, 1.2 W/cm2, duty ratio 30%) combined with ePFC5-loaded avidin/HVJ liposomes resulted in a decrease in cell viability at 1 h after irradiation to 43% of that of controls without ultrasound irradiation or liposomes. The cell viability was lower than that of cells treated with ultrasound irradiation with ePFC5-loaded avidin liposomes or ePFC5-loaded HVJ liposomes. This indicates that co-modification of liposome with avidin and HVJ envelope could enhance ultrasound-induced cell injury in the presence of ePFC5-loaded liposomes. |
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| Keywords: | Ultrasound Liposome Perfluorocarbon HVJ envelope Avidin Cancer cells |
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