Ultrasound-enhanced chemiluminescence tomography in biological tissue |
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| Affiliation: | 1. School of Civil Engineering and Surveying, Faculty of Health, Engineering and Sciences, University of Southern Queensland, Toowoomba, 4350 QLD, Australia;2. School of Mechanical and Electrical Engineering, Faculty of Health, Engineering and Sciences, University of Southern Queensland, Toowoomba, 4350 QLD, Australia;1. Fluorescence Imaging Group, Departamento de Física de Materiales C-04, Instituto Nicolás Cabrera, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain;2. Grupo de Fotônica e Fluidos Complexos, Instituto de Física, Universidade Federal de Alagoas, 57072-970 Maceió, Alagoas, Brazil;3. Instituto Ramón y Cajal de Investigación Sanitaria, Hospital Ramon y Cajal, Madrid 28034, Spain;1. Key Laboratory of Three Gorges Reservoir Region’s Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China;2. National Centre for International Research of Low-carbon and Green Buildings, Chongqing University, Chongqing 400045, China;3. Environmental monitoring station of Dadukou District, Chongqing, 400084, China;4. School of Environmental and Biological Engineering, Chongqing Technology and Business University, Chongqing 400067, China;1. Department of Physics Education, K.K. Education Faculty, Atatürk University, Erzurum 25240, Turkey;2. Department of Nanoscience & Nanoengineering, Graduate School of Natural and Applied Sciences, Ataturk University, Erzurum 25240, Turkey;3. Department of Physics, Science Faculty, Ataturk University, Erzurum 25240, Turkey;1. Center for Theragnosis, Korea Institute of Science and Technology (KIST), 39-1, Hawolgok-dong, Seongbuk-gu, Seoul 136-791, Republic of Korea;2. Department of Chemistry, Yonsei University, 50 Yonsei-ro, Seodaemungu, Seoul 120-749, Republic of Korea;3. Department of Material Science and Engineering, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul 151-744, Republic of Korea |
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| Abstract: | This paper reports ultrasound-assisted optical imaging of chemiluminescent probes in biological tissue. A focused low power ultrasound sonochemically enhances a peroxyoxalate chemiluminescence (CL) that involves indocyanine green (ICG) as luminescent pigments. By scanning the focus, it produces tomographic images of CL in scattering media. The authors demonstrate imaging using a slab of porcine muscle measuring 50 × 50 × 75 mm, in which a capsuled CL reagent is embedded at 25 mm depth. Spatial resolution of imaging and concentration characteristics of CL reagents to enhanced CL intensity are also studied to evaluate the potential for use in bio-imaging applications with exploring the CL enhancement mechanisms. CL enhancement ratio, defined as the ratio of ultrasonically enhanced CL intensity to the base intensity without ultrasound irradiation, was found to be constant even in varying ICG and oxidizer concentrations, implying to be applicable for quantitative determination of these molecules. |
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| Keywords: | Ultrasound-assisted Optical imaging Multimodal imaging Bio-imaging Sonochemistry Free radicals |
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