| SPECTRA PROPERTIES OF RAT LIVER MITOCHONDRIAL CHOLINE DEHYDROGENASE |
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| 引用本文： | 裘之华,林其谁. SPECTRA PROPERTIES OF RAT LIVER MITOCHONDRIAL CHOLINE DEHYDROGENASE[J]. 中国科学B辑(英文版), 1990, 0(8) |
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| 作者姓名： | 裘之华 林其谁 |
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| 作者单位： | Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC，Shanghai Institute of Biochemistry，Academia Sinica，Shanghai 200031，PRC |
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| 基金项目： | Project supported by the National Natural Science Foundation of China. |
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| 摘 要： | Choline dehydrogenase contains the prosthetic group FAD, non-haem iron and acid labile sulfur. However, the absorption spectra of the purified enzyme do not change after adding substrate. The reduced absorption spectra of choline dehydrogenase can only be determined after the addition of dithionite. Those choline dehydrogenases situated in the mitochondrial inner membrane can be reduced by substrate and exist in the reduced state. When cholate was used to solubilize the substrate-reduced choline dehydrogenase, the reduced spectra will gradually disappear. However, if solubilization is carried out under anaerobic conditions, the reduced spectra can be retained, suggesting that the solubilized choline dehydrogenase can use oxygen as an acceptor.
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SPECTRA PROPERTIES OF RAT LIVER MITOCHONDRIAL CHOLINE DEHYDROGENASE |
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| QIU ZHI-HUA LIN QI-SHUI. SPECTRA PROPERTIES OF RAT LIVER MITOCHONDRIAL CHOLINE DEHYDROGENASE[J]. Science in China(Chemistry), 1990, 0(8) |
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| Authors: | QIU ZHI-HUA LIN QI-SHUI |
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| Abstract: | Choline dehydrogenase contains the prosthetic group FAD, non-haem iron and acid labile sulfur. However, the absorption spectra of the purified enzyme do not change after adding substrate. The reduced absorption spectra of choline dehydrogenase can only be determined after the addition of dithionite. Those choline dehydrogenases situated in the mitochondrial inner membrane can be reduced by substrate and exist in the reduced state. When cholate was used to solubilize the substrate-reduced choline dehydrogenase, the reduced spectra will gradually disappear. However, if solubilization is carried out under anaerobic conditions, the reduced spectra can be retained, suggesting that the solubilized choline dehydrogenase can use oxygen as an acceptor. |
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| Keywords: | mitochondria choline dehydrogenase flavin adenine dinucleotide(FAD) |
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