EPR study of some irradiated food enzymes |
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| Authors: | O G Duliu M Ferdes O S Ferdes |
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| Institution: | 1. Department of Atomic and Nuclear Physics, M?gurele, University of Bucharest, P.O. Box MG-11, RO-76900, Bucharest, Romania
2. Plasma and Radiation Physics, Electron Accelerator Laboratory, M?gurele, National Institute for Lasers, P.O. Box MG-36, RO-76900, Bucharest, Romania
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| Abstract: | The EPR spectra of three types of amylases (microbial and fungal α-amylase E.C. 3.2.1.1; gluco-amylase E.C. 3.2.1.3) and one
type of pectinase irradiated with gamma-rays and 7 MeV electrons are presented and discussed. For all enzymes, a positive
correlation (r = 0.991 to 0.994) between the EPR signal amplitude and absorbed dose has been observed, while, between EPR spectra amplitudes
and enzyme in vitro activity, in terms of enzymatic activity a negative correlation (r = -0.987 to -0.995), has been noticed. These facts recommend that enzyme damage during irradiation can be monitored by means
of the free radicals detected by EPR spectroscopy.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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