A microcalorimetric study of the biologic effect of Mn(II) on Bacillus thuringiensis growth |
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| Authors: | Yao J Liu Y Gao Z T Liu P Sun M Qu S S Yu Z N |
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| Institution: | (1) Key Laboratory of Agricultural Microbiology of Chinese Agriculture Ministry, Huazhong Agricultural University, Wuhan, 430070, P. R. China;(2) Department of Chemistry, College of Chemistry and Molecular Science, Wuhan University, Wuhan, 430072, P. R. China |
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| Abstract: | A microcalorimetric technique based on the bacterial heat-output was explored to evaluate the effect of Mn(II) on Bacillus thuringiensis. The power-time curves of the growth metabolism of B. thuringiensis and the effect of Mn(II) on it were studied using an LKB-2277 BioActivity Monitor, ampoules method, at 28°C. For evaluation
of the results, the maximum peak-heat output power (P
max) in the growth phase, the growth rate constants (k), the log phase heat effects (Q
log ), and the total heat effect in 23 h (Q
T) for B. thuringiensis were determined. Manganese has been regarded as the essential biological trace element. Mn(II) of different concentration
have different effects on B. thuringiensis growth metabolism. High concentration (800-1600 μg mL-1) of Mn(II) can promote the growth of B. thuringiensis; low concentration (500-800 μg mL-1) can inhabit its growth.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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| Keywords: | Bacillus thuringiensis Mn(II) growth metabolism microcalorimetry |
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