Separation of Polar and Basic Compounds in Hydrophilic Interaction Pressurized CEC Using Diethylenetriaminopropyl Silica Monolithic Columns |
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Authors: | Fanggui Ye Zenghong Xie Xiaoping Wu Xucong Lin Guonan Chen |
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Affiliation: | (1) The Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian, 350002, China |
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Abstract: | A novel silica-based monolithic column possessing diethylenetriaminopropyl ligands for hydrophilic interaction pressurized capillary electrochromatography is described. The preparation of monolithic stationary phase was based on the individual silica matrix forming and subsequent chemical bonding. The triamino groups on the surface of the novel stationary phase generated a sustainable anodic electroosmotic flow under acidic conditions. A variety of neutral and basic analytes were used to evaluate the column performance. The monolithic silica stationary phase exhibited hydrophilic interaction chromatographic behavior toward neutral solutes. For basic tetracycline antibiotics, hydrophilic interaction as well as electrophoretic migration process with the monoliths was observed and peak tailing was avoided. |
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Keywords: | Column liquid chromatography Pressurized capillary electrochromatography Monolithic column Stationary phase Hydrophilic interaction |
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