DNA linearization through confinement in nanofluidic channels |
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Authors: | Nicholas Douville Dongeun Huh Shuichi Takayama |
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Institution: | (1) Department of Biomedical Engineering, University of Michigan, 2200 Bonisteel Blvd, Ann Arbor, MI 48109, USA;(2) Macromolecular Science and Engineering Center, University of Michigan, 2300 Hayward St., Ann Arbor, MI 48109, USA;(3) Present address: Vascular Biology Program, Department of Pathology and Surgery, Harvard Medical School and Children’s Hospital, Boston, MA 02115, USA |
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Abstract: | Stretching DNA has emerged as a vital process for studying the physical and biological properties of these molecules. Over
the past decade, there has been increasing research interest in utilizing nanoscale fluidic channels to confine and stretch
single DNA molecules. Nanofabricated systems for linearizing DNA have revealed new and important insights into the conformation
changes of DNA molecules. They also have emerged as innovative techniques for efficiently separating DNA molecules based on
size and for physically mapping genetic information along the genome. This review describes physical theories of DNA linearization,
current DNA stretching techniques based on nanofabricated channels, and breakthroughs resulting from the use of nanofluidic
channels for DNA linearization. |
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Keywords: | DNA linearization Nanochannels Confinement DNA stretching Nanofluidics Gene mapping |
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