Label-free detection of specific DNA sequence-telomere using unmodified gold nanoparticles as colorimetric probes |
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| Authors: | Yingying Qi Li Li Baoxin Li |
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| Institution: | aKey Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi’an 710062, China |
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| Abstract: | A simple and sensitive label-free colorimetric detection of telomere DNA has been developed. It was based on the color change of gold nanoparticles (AuNPs) due to DNA hybridization. UV–vis spectra and transmission electron microscopy (TEM) were used to investigate the change of AuNPs. Under the optimized conditions, the linear range for determination of telomere DNA was 5.7 × 10−13 to 4.5 × 10−6 mol/L. The detection limit (3σ) of this method has decreased to pico-molar level. |
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| Keywords: | Telomere DNA Hybridization Gold nanoparticles Colorimetry |
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