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Surface plasmon resonance immunosensor for cortisol and cortisone determination
Authors:Marco Frasconi  Monica Mazzarino  Francesco Botrè  Franco Mazzei
Institution:(1) Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy;(2) Laboratorio Antidoping Federazione Medico Sportiva Italiana, Largo Giulio Onesti 1, 00197 Rome, Italy
Abstract:In this paper, we present a surface-plasmon-resonance-based immunosensor for the real-time detection of cortisol and cortisone levels in urine and saliva samples. The method proposed here is simple, rapid, economic, sensitive, robust, and reproducible thanks also to the special features of the polycarboxylate-hydrogel-based coatings used for the antibody immobilization. The sensor surface displays a high level of stability during repeated regeneration and affinity reaction cycles. The immunosensor shows high specificity for cortisol and cortisone; furthermore, no significant interferences from other steroids with a similar chemical structure have been observed. The suitability of the hydrogel coating for the prevention of nonspecific binding is also investigated. A good correlation is noticed between the results obtained by the proposed method and the reference liquid chromatography/tandem mass spectrometry method for the analysis of cortisol and cortisone in urine and saliva samples. Standard curves for the detection of cortisol and cortisone in saliva and urine are characterized by a detection limit less than 10 μg l−1, sufficiently sensitive for both clinical and forensic use. MediaObjects/216_2009_2914_Figa_HTML.gif Application of a newly developed SPR immunosensor for the measurement of cortisol in anti-doping analysis
Keywords:Cortisol  Cortisone  SPR  Immunosensor  Antidoping
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