Surface plasmon resonance immunosensor for cortisol and cortisone determination |
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Authors: | Marco Frasconi Monica Mazzarino Francesco Botrè Franco Mazzei |
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Institution: | (1) Dipartimento di Chimica e Tecnologie del Farmaco, Sapienza Università di Roma, Piazzale Aldo Moro 5, 00185 Rome, Italy;(2) Laboratorio Antidoping Federazione Medico Sportiva Italiana, Largo Giulio Onesti 1, 00197 Rome, Italy |
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Abstract: | In this paper, we present a surface-plasmon-resonance-based immunosensor for the real-time detection of cortisol and cortisone
levels in urine and saliva samples. The method proposed here is simple, rapid, economic, sensitive, robust, and reproducible
thanks also to the special features of the polycarboxylate-hydrogel-based coatings used for the antibody immobilization. The
sensor surface displays a high level of stability during repeated regeneration and affinity reaction cycles. The immunosensor
shows high specificity for cortisol and cortisone; furthermore, no significant interferences from other steroids with a similar
chemical structure have been observed. The suitability of the hydrogel coating for the prevention of nonspecific binding is
also investigated. A good correlation is noticed between the results obtained by the proposed method and the reference liquid
chromatography/tandem mass spectrometry method for the analysis of cortisol and cortisone in urine and saliva samples. Standard
curves for the detection of cortisol and cortisone in saliva and urine are characterized by a detection limit less than 10 μg l−1, sufficiently sensitive for both clinical and forensic use.
Application of a newly developed SPR immunosensor for the measurement of cortisol in anti-doping analysis |
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Keywords: | Cortisol Cortisone SPR Immunosensor Antidoping |
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