| 中等规模多肽分子的2DNOESY实验问题 |
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| 引用本文： | 张一兵,余亦年.中等规模多肽分子的2DNOESY实验问题[J].波谱学杂志,1994,11(2):219-222. |
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| 作者姓名： | 张一兵 余亦年 |
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| 作者单位： | 中国科学院上海有机化学研究所生命有机化学国家重点实验室, 上海 200032 |
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| 摘 要： | 由于中等规模多肽在水溶液中运动的特殊性(ω0τc≈1),2DNOESY谱上往往得不到相关讯息(采用合理的混合时间),而降低温度的余地也很小·本工作将少量氘代甲醇加到水溶液中,并将实验温度降到零下,实验取得成功。例如从一个九肽和一个十一肽的NOESY实验得到了很多残基内和残基间的NOESY相关讯息,足以作出归属并进一步计算质子间距离.
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| 关 键 词： | 2D NOESY 中等规模多肽 |
| 收稿时间： | 1993-01-04 |
2D NOESY EXPERIMENTAL PROBLEMS OF MEDIUM-SIZED PEPTIDES |
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| Zhang Yibing,Yu Yihua,Shen Wei,Wang Qiwen.2D NOESY EXPERIMENTAL PROBLEMS OF MEDIUM-SIZED PEPTIDES[J].Chinese Journal of Magnetic Resonance,1994,11(2):219-222. |
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| Authors: | Zhang Yibing Yu Yihua Shen Wei Wang Qiwen |
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| Institution: | State Key Laboratory or Bio-organic and Natural products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032 |
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| Abstract: | The main difficulty in 2D NOESY of mcdium-sized peptdes is lacking of cross-peaks, espccially in the region related with NH, For solving this problem, these authors use a mixed solvent,CD3OD/H2O, to dissolve peptides,which allows NMR measurements at low temperature.The increased viscosity of solution and the correlation times by low-temperature measurements made a lot of intra-and inter-residue NOES observable, which helped us unambiguously complete the assigmnents of a nonapeptide and an undccapcptide, and further in calculation of H-Hdistances. |
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| Keywords: | 2D NOESY Medium-sized peptides |
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