Validation Method for the Determination of Sulfonamide Residues in Bovine Milk by HPLC |
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Authors: | Yin-Liang Wu Cun Li Yong-Jun Liu Jian-Zhong Shen |
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Institution: | (1) College of Veterinary Medicine, China Agricultural University, Beijing, 100094, People’s Republic of China;(2) Quality Control and Inspection Center for Domestical Animal Products of MOA, Beijing, 100026, People’s Republic of China;(3) College of Agriculture, Hebei University of Engineering, Hangdang, 056038, People’s Republic of China |
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Abstract: | A simple, rapid, sensitive and reliable high-performance liquid chromatographic method for the simultaneous determination
of eight sulfonamides (SAs) in bovine milk was developed (sulfadiazine, sulfathiazole, sulfamethazine, sulfamethoxypyridazine,
sufamonomethoxine, sulfamethoxazole, sulfadimethoxine and sulfaquinoxaline) in bovine milk was developed. Samples were prepared
by extraction with ethyl acetate and cleaning-up with an anion solid-phase extraction (SPE) column. Analytical separation
was performed on an Inertsil ODS-3 column with photodiode-array detection at 270 nm under gradient condition. The whole procedure
was evaluated according to the European Commission Decision 2002/657/EC. Specificity, decision limit (CCα), detection capacity
(CCβ), trueness and precision were determined during the validation process. It was found that the analytes were isolated
from spiked samples with good recoveries between 70.5 and 89.0%. The used analytical conditions allow to successively separating
all the tested sulfonamides with good limit of detection between 0.8 and 1.5 μg L−1. |
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Keywords: | Column liquid chromatography Bovine milk Sulfonamides residues |
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