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An investigation into the use of semi-microbore reverse-phase 3 μm columns in HPLC |
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| Authors: | G D Reed C R Loscombe |
| Institution: | (1) Laboratory of the Government Chemist, Cornwall House, Stamford Street, SE1 9NQ London, U. K. |
| Abstract: | Summary The preparation and use of semi-microbore (2mm i.d.), reverse-phase columns in HPLC has been investigated and they have been compared with 5 mm bore analytical columns. Injection valve design, detector cell volume, column loading and column linking are considered for both semi-microbore and analytical systems. Mobile phase flow-rate, detector time constant and column lifetime are also evaluated for small-bore systems. |
| Keywords: | Column liquid chromatography Semi-microbore columns Reverse-phase 3 |
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