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| 紫外-可见光谱法研究细胞色素C活性中心金属离子与硫酸铜的相互作用 | |
| 引用本文： | 贾颖萍,郑学仿,胡皆汉. 紫外-可见光谱法研究细胞色素C活性中心金属离子与硫酸铜的相互作用[J]. 光谱学与光谱分析, 2001, 21(1): 54-56 |
| 作者姓名： | 贾颖萍 郑学仿 胡皆汉 |
| 作者单位： | 1. 大连大学化学与化学工程系, 2. 大连大学化学与化学工程系;大连理工大学化工学院生物工程系 3. 中国科学院大连化学物理研究所, |
| 摘 要： | 应用紫外－可见吸收光谱法研究细胞色素C（Fecyt-C)活性中心铁离在pH7.1的磷酸缓冲液中与无机CuSO4的直接相互作用。结果发现：CuSO4中的Cu（Ⅱ）与Fecyt-C中的Fe(Ⅱ）有交换作用，形成部分的Cucyt-C，并且随着外加CuSO4量的增加，这种相互作用增强，首次证实了细胞色素C这种直接相互作用的存在。 |
| 关 键 词： | 细胞色素C 硫酸铜 活性中心铁离子 紫外－可见吸收光谱 相互作用 |
| 修稿时间： | 2000-05-12 |
Interaction between the Metal Ion in the Active Site of Cytochrome C and Copper Sulfate by Ultraviolet Spectroscopy |
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| Yingping JIA,Xuefang ZHENG , and Jiehan HU. Interaction between the Metal Ion in the Active Site of Cytochrome C and Copper Sulfate by Ultraviolet Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2001, 21(1): 54-56 | |
| Authors: | Yingping JIA Xuefang ZHENG Jiehan HU |
| Affiliation: | Department of Chemistry and Chemical Engineering, Dalian University, 116622 Dalian. |
| Abstract: | The direct interaction between the metal ion in the active center of cytochrome C (Fecyt-C) and inorganic copper sulfate in pH 7.1 phosphate has been studied by UV spectrometry. As a result, part of the metal ion Fe(II) in Fecyt-C was replaced by Cu(II) and the corresponding cytochrome C derivatives Cucyt-C were produced, in addition, the interaction was increased with amount of copper sulfate added. The direct interaction between the CuSO4 and metal ion of cytochrome C was verified for the first time. |
| Keywords: | Cytrochrome C Copper sulfate Ultraviolet absorption spectroscopy |
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