CIRCULAR DICHROISM SPECTRA OF SYSTEM I PARTICLES FROM NORMAL CHLOROPLASTS AND CAROTENOID-DEFICIENT MUTANTS OF MAIZE |
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| Authors: | A. GARAY,S. DEMETER,K. KOVÁ CS,G. HORVÁ TH,Á GNES FALUDI-DÁ NIEL |
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| Affiliation: | Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary |
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| Abstract: | Abstract— Circular dichroism (CD) spectra were studied in System I particles prepared by digitonin fragmentation of chloroplasts of normal maize and carotenoid-deficient mutants. CD bands of pigment molecules were found to be higher in particles than in solutions. This phenomenon was most pronounced for the particles containing the carotenoid composition of normal chloroplast lamellae. The CD signal of chlorophylls bound to particles of carotenoid-deficient chloroplasts was closer to that found in solutions. This indicates that aggregation and/or binding of chlorophylls in carotenoid-deficient mutants may be less extensive than in normal chloroplasts. |
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