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Visualisation of biopolymer mixtures using confocal scanning laser microscopy (CSLM) and covalent labelling techniques |
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| Authors: | Fred van de Velde Fanny Weinbreck Marijke W. Edelman Erik van der Linden R. Hans Tromp |
| Affiliation: | a Wageningen Centre for Food Sciences, P.O. Box 557, 6700 AN, Wageningen, Netherlands b NIZO Food Research, Product Technology Department, P.O. Box 20, 6710 BA, Ede, Netherlands c Laboratory of Food Physics, Department of Agrotechnology and Nutrition Sciences, Wageningen University, Bomenweg 2, 6703 HD, Wageningen, Netherlands |
| Abstract: | Confocal scanning laser microscopy (CSLM) has been used to study the behaviour of mixtures of proteins, gelatine, whey proteins and β-lactoglobulin, and polysaccharides, dextran, gellan gum, carrageenan, gum Arabic, and starch. CSLM proved to be a suitable technique to visualise the microstructure of these (phase separated) mixtures in two and three-dimensional images. Contrast through fluorescence is obtained either by covalent labelling (polysaccharides and proteins) or non-covalent labelling (proteins and starch). Double and triple labelling allows the visualisation of individual components in a complex mixture of biopolymers. |
| Keywords: | Visualisation Biopolymer mixtures Confocal scanning laser microscopy Covalent labelling |
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