HACACO revisited: residual dipolar coupling measurements and resonance assignments in proteins |
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Authors: | Cicero Daniel O Contessa Gian Marco Paci Maurizio Bazzo Renzo |
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Affiliation: | Department of Chemical Science and Technology, University of Rome "Tor Vergata," Italy. |
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Abstract: | The revisited version of the HACACO experiment here presented, is more robust and straightforward to implement and continues to be, to a greater extent, a convenient tool for protein backbone resonance assignment. Additionally, it turns out to be a sensitive and accurate method to measure C(alpha)-H(alpha) residual dipolar couplings (RDCs). The performance of our new pulse scheme for measurement of RDCs was tested on two proteins with different secondary structures: one characterized by a high beta-sheet content, the second dominated by the presence of alpha-helices. In both examples the new method provided significantly more accurate data, compared to all previously published 3D techniques. |
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Keywords: | HACACO Residual dipolar couplings Protein Solution NMR |
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