| 膜材性质及制备方法调控下的脂质体负载干扰素的研究 |
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| 引用本文： | 王妍,张水华,李云富,李久立,曹淑桂.膜材性质及制备方法调控下的脂质体负载干扰素的研究[J].高等学校化学学报,2006,27(10):1986-1990. |
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| 作者姓名： | 王妍 张水华 李云富 李久立 曹淑桂 |
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| 作者单位： | 吉林大学分子酶学工程教育部重点实验室,长春,130023;深圳市海王英特龙生物技术股份有限公司,深圳,518054;深圳市海王英特龙生物技术股份有限公司,深圳,518054;吉林大学分子酶学工程教育部重点实验室,长春,130023 |
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| 摘 要： | 依据干扰素(IFN)分子、磷脂分子本身的理化性质和结构特点, 分别用三种制备方法, 以四种脂质体为膜材, 制备IFN脂质体, 考察了不同膜材、不同制备方法对脂质体粒径及包封率的影响. 结果表明, 以二肉豆蔻酰胆碱和二棕榈酰磷脂酰胆碱复合材料为主要膜材, 采用薄膜蒸发法制备的IFN脂质体有良好的稳定性, 60 d内其粒径可以保持在200~350 nm, 包封率可保持30%~40%.
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| 关 键 词： | 脂质体 干扰素 薄膜蒸发法 粒径分析 |
| 文章编号： | 0251-0790（2006）10-1986-05 |
| 收稿时间： | 01 17 2006 12:00AM |
| 修稿时间： | 2006-01-17 |
Research of IFN-encapsulated Liposomes Regulated by Lecithin Characteristics and Preparation Methods |
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| WANG Yan,ZHANG Shui-Hua,LI Yun-Fu,LI Jiu-Li,CAO Shu-Gui.Research of IFN-encapsulated Liposomes Regulated by Lecithin Characteristics and Preparation Methods[J].Chemical Research In Chinese Universities,2006,27(10):1986-1990. |
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| Authors: | WANG Yan ZHANG Shui-Hua LI Yun-Fu LI Jiu-Li CAO Shu-Gui |
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| Institution: | Key Laborabory for Medcular Enzymology &; Engineering, Ministry of Education, Jilin University, Changchun 130023, China; ;Shenzhen Neptunus Interlong Bio-technique Holdings Co., LTD, Shenzhen 518054, China |
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| Abstract: | According to the physical and chemical characteristics of interferon and Lecithin, we selected four kinds of lecithin compounds as the liposomal membrane aterials, prepared liposomes by three methods, investigated liposomal shape, size distribution and the entrapment efficiency(EE) for a 60 d period to explore their influence factors of stability. The result shows the liposome made from DMMPC+DPPC+SA by film evaporation method has a good EE and size stability for 2 months. The size range is from 200 nm to 300 nm, the EE range is from 30%—40%. |
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| Keywords: | Liposome Interferon Film evaporation method Size distribution |
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