| 人淋巴瘤白血病细胞系Molt-4的31P核磁共振研究 |
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| 引用本文： | 黄荣清,杜泽函.人淋巴瘤白血病细胞系Molt-4的31P核磁共振研究[J].波谱学杂志,1996,13(2):107-112. |
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| 作者姓名： | 黄荣清 杜泽函 |
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| 作者单位： | 1. 军事医学科学院放射医学研究所;2. 国家生物医学分析中心;3. 基础医学研究所, 北京 100850 |
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| 摘 要： | 用31P核磁共振(NMR)观察了人淋巴瘤白血病细胞系Molt-4的31P谱.NMR测试温度为37℃、20℃和4℃时,随着测试时间增长,细胞31P谱中ATP峰降低,无机磷峰升高,细胞31P谱的变化随测试温度降低依次减缓.实验温度为4℃,从收集细胞至开始NMR累加在30min内,NMR累加在60min内,获得活细胞的稳定的31P谱.根据细胞内无机磷的化学位移数值,对细胞内pH值进行了测定.
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| 关 键 词： | 31P核磁共振 Molt-4细胞 pH |
| 收稿时间： | 1995-07-03 |
31P NUCLEAR MAGNETIC RESONANCE STUDIES OF HUMAN LYMPHOMA CELL LINE MOLT-4 |
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| Huang Rongqing,Du Zehan,Yang Zhigang and Feng Rui.31P NUCLEAR MAGNETIC RESONANCE STUDIES OF HUMAN LYMPHOMA CELL LINE MOLT-4[J].Chinese Journal of Magnetic Resonance,1996,13(2):107-112. |
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| Authors: | Huang Rongqing Du Zehan Yang Zhigang and Feng Rui |
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| Institution: | 1. Institute of Radiation Medicine;2. National Centre of Biomedical Analysis;3. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850 |
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| Abstract: | 31P nuclear magnetic resonance (NMR) spectra of human lymphoma cell line Molt-4 were observed by using 31P NMR. The decrease in ATP levels and simultaneous increase in inorganic phosphate (Pi) levels occurred as the time of NMR experiment increased under temperatures of 37℃, 20℃ and 4℃.The changes in 31P spectra of cells slow down sequentially following the descent of NMR measurement temperature. Stable 31P spectrum of living cells was obtained when accumulation of data was started within 30min after the harvest of the cells and finished within 60 min at 4℃.Intracellular pH of Molt-4 cells was measured based on the chemical shift of Pi peak. |
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| Keywords: | ?31)P NMR Molt-4 cells pH |
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