Antioxidant peptides from <Emphasis Type="Italic">Cicer arietinum</Emphasis> of Xinjiang,China |
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| Authors: | A Yili Q L Ma Q Y Lv Y H Gao B Zhao O N Veshkurova Sh I Salikhov H A Aisa |
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| Institution: | 1.Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry,Academy of Sciences of the People?s Republic of China,Urumqi,P. R. China;2.A. S. Sadykov Institute of Bioorganic Chemistry,Academy of Sciences of the Republic of Uzbekistan,Tashkent,Republic of Uzbekistan |
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| Abstract: | Four peptides of molecular weights 1.148, 4.68, 5.41, and 9.086 kDa with antioxidant activity were isolated from chickpea
sprout using two-step ion-exchange chromatographic and HPLC techniques for the first time. The partial N-terminal amino acid
sequences of the peptide named YZDBCM-1 (9.086 kDa) was determined as H2N1-15-H2N-Ala-Ile-Thr-Cys-Gly-Arg-Val-Ser-Ala-Ala-Leu-Ala-Pro-Pro-Leu using the Edman automated sequencing apparatus, which was a
new peptide rich in alanine. It was shown that the antioxidant activity of the peptide YZDBCM-1 was IC50 156.2 μg/mL (17.2 μmol/L) to the free radical of ABTS. |
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