Determination of Trace Cholinergic Compounds and the Evaluation of Their Relative Receptor Activity with the Use of a Receptor Biosensor and an Ion-Selective Choline Electrode |
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Authors: | E B Nikol'skaya B N Kormilitsyn L I Kugusheva |
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Institution: | (1) Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, pr. Morisa Toreza 44, St. Petersburg, 194223, Russia |
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Abstract: | A comparative assessment of the analytical capabilities of a tensoresistive biosensor based on muscarinic acetylcholine receptors and a potentiometric choline sensor was performed. It was found that the receptor biosensor is promising for the determination of ultratrace amounts of cholinergic agents with a detection limit of 10–7–10–10 M. The selectivity of the potentiometric choline sensor is equal to the selectivity of the receptor biosensor; however, the sensitivity of the former is lower by two orders of magnitude. Nevertheless, it can be successfully used for evaluating the relative receptor activity of cholinergic agents. |
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