Rapid determination of levofloxacin in pharmaceuticals and biological fluids using a new chemiluminescence system |
| |
| Authors: | Xiaodong Shao Ying Li Yangqin Liu Zhenghua Song |
| |
| Institution: | 1.CNPC Tubular Goods Research Institute,Xi’an,P.R. China;2.Xi’an Thermal Power Research Institute Co. Ltd,Xi’an,P.R. China;3.Northwest University,Xi’an,P.R. China |
| |
| Abstract: | A novel chemiluminescence method for the determination of levofloxacin is presented, which is based on the inhibitory effect
of levofloxacin on the chemiluminescence reaction between luminol and myoglobin in a flow-injection system. The decrement
of chemiluminescence intensity is linear with the logarithm of levofloxacin concentration over the range from 0.07 to 100.0
ng/mL (r
2 = 0.9994), with the detection limit of 0.02 ng/mL (3σ). At a flow rate of 2.0 mL/min, a complete analytical process could
be performed within 0.5 min, including sampling and washing, with a relative standard deviation of less than 3.0% (n = 5). The proposed procedure was applied successfully to the determination of levofloxacin in pharmaceutical preparations,
human urine and serum without any pretreatment procedure. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
