SERS as tool for the analysis of DNA-chips in a microfluidic platform |
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Authors: | Katharina K Strelau Robert Kretschmer Robert Möller Wolfgang Fritzsche Jürgen Popp |
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Institution: | 1. Jenaer Biochip Initiative, Institute of Physical Chemistry, Friedrich-Schiller-University Jena, Helmholtzweg 4, 07745, Jena, Germany 2. Institute of Photonic Technology, Albert-Einstein-Stra?e 9, 07745, Jena, Germany
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Abstract: | A sequence-specific detection method of DNA is presented combining a solid chip surface for immobilisation of capture DNAs
with a microfluidic platform and a readout of the chip based on SERS. The solid chip surface is used for immobilisation of
different capture DNAs, where target strands can be hybridised and unbound surfactants can be washed away. For the detection
via SERS, short-labelled oligonucleotides are hybridised to the target strands. This technique is combined with a microfluidic
platform that enables a fast and automated preparation process. By applying a chip format, the problems of sequence-specific
DNA detection in solution phase by means of SERS can be overcome. With this setup, we are able to distinguish between different
complementary and non-complementary target sequences in one sample solution. |
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Keywords: | |
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