Purification and Characterization of Enterocin LR/6, a Bacteriocin from <Emphasis Type="Italic">Enterococcus faecium</Emphasis> LR/6 |
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Authors: | Manoj Kumar Santosh Kumar Tiwari Sheela Srivastava |
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Institution: | (1) Department of Genetics, University of Delhi South Campus, New Delhi, 110021, India; |
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Abstract: | Enterocin LR/6, a bacteriocin obtained from the culture filtrate of Enterococcus faecium strain LR/6, has been purified to homogeneity using ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration,
and checked on reverse-phase high-performance liquid chromatography. It is active at high temperatures (boiling as well as
autoclaving) and over a wide range of pH (2.0–8.0). Also, it is sensitive to a number of proteolytic enzymes but is stable
in the presence of surfactants and organic solvents. The protein could be stored at least up to 1 year at low temperatures
(4 °C and −20 °C) without any loss of activity. The N-terminal sequence of enterocin LR/6 showed no homology with known enterocins
or other bacteriocins present in the database, suggesting it to be a novel enterocin. Matrix-assisted laser desorption/ionization-time-of-flight
mass spectrometry and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed its mass to be ∼6.1 kDa.
It showed a bactericidal mode of action against indicator strain, Micrococcus luteus. |
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Keywords: | |
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