An Effective and Simplified Fed-Batch Strategy for Improved 2,3-Butanediol Production by <Emphasis Type="BoldItalic">Klebsiella oxytoca</Emphasis> |
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Authors: | Zhi-Kui Nie Xiao-Jun Ji He Huang Jun Du Zhi-Yong Li Liang Qu Qi Zhang Ping-Kai Ouyang |
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Institution: | (1) State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People’s Republic of China; |
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Abstract: | Substrate concentration in 2,3-butanediol (2,3-BD) fermentation could not be controlled well in traditional feeding strategies,
such as constant, impulse, and exponential feeding strategies. In the present study, fermentative 2,3-BD production by Klebsiella oxytoca was investigated under different batch and fed-batch strategies. The glucose-feedback fed-batch strategy was proved to be
not effective for economical 2,3-BD production for the inability of timely feeding, leading that the bacteria reused 2,3-BD
as carbon source for cell growth. Based on the phenomena that the byproducing acids caused the pH declining and the requirement
of maintaining the pH at a proper level for both cell growth and 2,3-BD accumulation, an improved strategy of pH-stat fed-batch
culture with glucose and sodium hydrate fed at the same time was established. Thus, the residual glucose concentration could
be controlled through the adjustment of pH automatically. At last, efficient 2,3-BD production was fulfilled under this fed-batch
strategy, and the highest 2,3-BD concentration, productivity, and yield were 127.9 g/l, 1.78 g/(l•h), and 0.48 g/g (2,3-BD/glucose),
respectively, compared to 98.5 g/l, 1.37 g/(l•h), and 0.43 g/g obtained in glucose-feedback fed-batch strategy. This feeding
strategy was simple and easy to operate and could be feasible for industrial 2,3-BD production in the future. |
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