Synthesis of Functionalized ZnSe Nanoparticles and Their Applications in the Determination of Bovine Serum Albumin |
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Authors: | Li Li Fenfen Zhang Yaping Ding Yaping Wang Lulu Zhang |
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Institution: | (1) Department of Chemistry, Shanghai University, Shanghai, 200444, PR China |
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Abstract: | Luminescent quantum dots (QDs)-semiconductor nanocrystals were promising alternative to organic dyes for fluorescence-based
applications. In this paper, we developed procedures to use mercaptoacetic acid (MAA) to modify ZnSe nanoparticles and made
the nanoparticles to be soluble for the quantitative and selective determination of bovine serum albumin (BSA). Maximum fluorescence
intensity was produced at pH 7.0, with excitation and emission wavelengths at 242 and 348 nm, respectively. Under optimal
conditions, the straight line equation: △
F = 0.38 + 0.34 C (μg/ml) was found between the relative fluorescence intensity and the concentration of BSA in the range of
9.6–124.8 μg/ml, and the limit of detection was 2 μg/ml. |
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Keywords: | Quantum dots Spectrofluorimetric detection ZnSe nanoparticles Bovine serum albumin |
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