| 薏苡多糖的提取、分离与纯化工艺研究 |
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| 引用本文： | 李厚聪,刘圆,袁玮.薏苡多糖的提取、分离与纯化工艺研究[J].西南民族学院学报(自然科学版),2009,35(2):278-282. |
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| 作者姓名： | 李厚聪 刘圆 袁玮 |
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| 作者单位： | 西南民族大学少数民族药物研究所,四川成都610041 |
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| 基金项目： | 国家科技支撑计划重点项目，四川省青年基金，国家中医药管理局项目 |
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| 摘 要： | 目的：建立薏苡非种仁部位中多糖的提取、分离和纯化工艺．方法：利用正交设计优化薏苡多糖的提取工艺，用Sevag法和三氯乙酸法脱蛋白．结果：正交设计优选薏苡多糖的提取工艺为12倍量的水提取2次，每次1h；Sevag法和三氯乙酸法均能有效地除去多糖中的蛋白质．结论：本实验为薏苡多糖的提取分离的条件提供了参考，为薏苡的进一步开发研究提供依据．
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| 关 键 词： | 薏苡 根茎叶 多糖 |
The extraction, separation and purification study of polysaccharide on Coix lacryma-jobi L. var. mayuen (Roman.) Stapf |
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| LI Hou-cong,LIU Yuan,YUAN Wei.The extraction, separation and purification study of polysaccharide on Coix lacryma-jobi L. var. mayuen (Roman.) Stapf[J].Journal of Southwest Nationalities College(Natural Science Edition),2009,35(2):278-282. |
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| Authors: | LI Hou-cong LIU Yuan YUAN Wei |
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| Institution: | (Ethnic Pharmaceutical Institute of Southwest University for Nationalities, Chengdu 610041, P.R.C.) |
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| Abstract: | Objective： To establish the extraction, separation and purification technology of the polysaccharide from the root, stem and leaf in C. acryrna-jobi L. var. mayuen （Roman.） Stapf. Methods： The orthogonal design, Sevag and trichloroacetic acid method are adopted. Results： The best extraction condition is 12 times with water extracted twice and 1 h each time. Both Sevag and trichloroacetic acid method could remove its protein. Conclusion： This experiment establishes the foundation of further research,on C. lacryma-jobi L. var. mayuen （Roman.） Stapf. |
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| Keywords: | Clacryma-jobi L var mayuen （Roman ） Stapf root Stem and leaf polysaccharide |
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