Characteristics of the 5S rRNA from cotton seeds

The individuality of the 5S rRNA from cotton seeds has been determined by the production of a single narrow band on gel electrophoresis and by the characteristic UV spectrum with values of the ratios E₂₆₀/E₂₃₀ and E₂₆₀/E₂₈₀ of 2.03–2.1 and 2.08–2.14, respectively. The melting curve of the 5S rRNA has been obtained and a total hyperchromic effect of 25% has been calculated. The nucleotide composition of the 5S rRNA has been established by determining the amount of nucleotides in an alkaline hydrolysate (22.6% of AMP; 30.2% of GMP; 22.6% of UMP; and 24.6% of CMP). The nucleotides were identified from the position of their appearance in the elution profile, from their UV spectra in 0.1 N HC1 and 0.1 KOH, and also by one- and two-dimensional chromatography of the nucleotides obtained, in the presence of markers.Institute of the Chemistry of Plant Substances of the Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 817–821, November–December, 1980.