| 寡糖衍生化及基质辅助激光解吸电离飞行时间质谱分析方法研究 |
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| 引用本文： | 韩欢欢,马岩,王璐,张万军,卫军营,张养军,钱小红.寡糖衍生化及基质辅助激光解吸电离飞行时间质谱分析方法研究[J].分析化学,2010,38(3). |
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| 作者姓名： | 韩欢欢 马岩 王璐 张万军 卫军营 张养军 钱小红 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所,北京蛋白质组研究中心,蛋白质组学国家重点实验室,北京,102206 |
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| 基金项目： | 国家重点基础研究规划项目(Nos.2006CB910801,2006CB910803,2007CB914104);;国家高技术研究发展计划项目(No.2006AA02A308);;国家自然科学基金项目(Nos.30621063,20635010,20735005,20875101);;国家重点实验室自主课题(No.2008ZX10207)资助项目 |
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| 摘 要： | 为提高中性寡糖在基质辅助激光解吸电离飞行时间质谱(MALDI-TOF)中的检测灵敏度,建立了以1-(4-氰基苯基)-4-哌啶碳酰肼(CPH)为衍生化试剂对寡糖的标记方法。寡糖的还原端与CPH的酰肼基团反应生成腙,使得寡糖被CPH标记,衍生物以MALDI-TOF质谱进行分析。结果表明:在反应温度95℃,醋酸浓度为0.125%(V/V),CPH过量100倍的条件下,衍生产率可达最大,并且CPH衍生可使中性寡糖在MALDI-TOF质谱中的检测灵敏度提高10倍。本方法简便快速,灵敏度高,适合微量寡糖链的质谱分析。
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| 关 键 词： | 基质辅助激光解吸电离飞行时间质谱 1-（4-氰基苯基）-4-哌啶碳酰肼 中性寡糖 |
Derivatization of Oligosaccharides and Analysis by Matrix-assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry |
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| HAN Huan-Huan,MA Yan,WANG Lu,ZHANG Wan-Jun,WEI Jun-Ying,ZHANG Yang-Jun,QIAN Xiao-Hong.Derivatization of Oligosaccharides and Analysis by Matrix-assisted Laser Desorption/Ionization-Time of Flight-Mass Spectrometry[J].Chinese Journal of Analytical Chemistry,2010,38(3). |
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| Authors: | HAN Huan-Huan MA Yan WANG Lu ZHANG Wan-Jun WEI Jun-Ying ZHANG Yang-Jun QIAN Xiao-Hong |
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| Institution: | State Key Lab of Proteomics;Beijing Proteome Research Center;Beijing 102206; Beijing Institute of Radiation Medicine;Beijing 100850 |
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| Abstract: | A derivatization method for the determination of oligosaccharides with high sensitivity by matrixassisted laser desorption/ionization-time of flight-mass spectrometry( MALDI-TOF-MS) based on a labeling regent 1-( 4-Cyanophenyl)-4-piperidinecarbohydrazide( CPH) was proposed. The hydrazones were generated by the reaction of the reducing ends of oligosaccharides and the hydrazide of CPH. The derivatives were directly analyzed by MALDI-TOF-MS. Reaction conditions such as reaction temperature,concentration of ac... |
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| Keywords: | Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry 1-(4-Cyanophenyl)-4-piperidinecarbohydrazide Neutral oligosaccharides |
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