Absorption-based highly sensitive and reproducible biochemical oxygen demand measurement method for seawater using salt-tolerant yeast Saccharomyces cerevisiae ARIF KD-003 |
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Authors: | Nakamura Hideaki Mogi Yotaro Hattori Hisashi Kita Yutaka Hattori Daisuke Yoshimura Aki Karube Isao |
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Affiliation: | aSchool of Bionics, Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan1;bResearch Center of Advanced Bionics (RCAB), National Institute of Advanced Industrial Science and Technology (AIST), Tokyo University of Technology, 1404-1 Katakura, Hachioji, Tokyo 192-0982, Japan |
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Abstract: | Salt-tolerant yeast Saccharomyces cerevisiae ARIF KD-003 was applied to highly sensitive and reproducible absorbance-based biochemical oxygen demand (BODAB-ScII) measurement for seawater. In the previous work, we have studied the BODAB-ScI method using normal Baker's yeast S. cerevisiae, and the excellent feature of the Baker's yeast as uniformly sustainable in solution could successfully be utilized. However, the BODAB-ScI responses were disappeared by the existence of chloride ion as well as seawater. In the present method, uniformity in solution was also observed with S. cerevisiae ARIF KD-003, and salt-tolerance of the yeast was observed even in saturate concentration of sodium chloride. Next, characterizations of the influences of pH and incubation temperature were investigated. After optimum conditions were obtained, two calibration curves were made between 0.33 and 22 mg O2 L−1 BOD using standard solution of glucose glutamic acid (GGA) or mixture of GGA and artificial seawater. Then, excellent reproducibility as the averages of relative standard deviation (R.S.D.av) in two calibration curves (nine points each) was successfully obtained at 1.10% at pure water or 1.03% at artificial seawater standard, respectively. In addition, the 3σ lower detection limit was calculated to be 0.07 mg O2 L−1 BOD, and 0.11 mg O2 L−1 BOD was experimentally detected by increase of the sample volume at 1.5-folds. The storage stability of the S. cerevisiae ARIF KD-003 was obtained at least 4 weeks. |
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Keywords: | Biochemical oxygen demand Highly sensitive Artificial seawater Salt-tolerant yeast Saccharomyces cerevisiae ARIF KD-003 Shirakami-Kodama-Kobo™ |
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