Cationic amylopectin derivatives as additives for analysis of proteins in capillary electrophoresis |
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Authors: | Kato Masaru Imamura Eriko Sakai-Kato Kumiko Nakajima Tohru Toyo'oka Toshimasa |
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Affiliation: | Department of Analytical Chemistry, School of Pharmaceutical Sciences and COE Program in the 21st Century, University of Shizuoka, Shizuoka, Japan. |
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Abstract: | Positively charged amylopectin, which is a major constituent of cationic starch, was used to modify the inner surface of fused-silica capillaries by addition to the running solution, which was subsequently employed in CE. Capillaries filled with cationic amylopectin derivatives were shown to generate a stable reversed EOF in the investigated range of pH 4-8. Among the additives studied, quaternary ammonium amylopectin derivatives with high amino and low hydroxypropyl groups showed fast electroosmotic mobility and very effectively suppressed the adsorption of proteins. The run-to-run and batch-to-batch repeatability of the procedures were satisfactory with RSDs of 0.5% and 2.4%, respectively. A basic protein, alpha-chymotrypsinogen, migrated within 6 min and the theoretical plate number of it reached 560 000 plates/m. |
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Keywords: | Amylopectin CE Microchip electrophoresis Protein Separation Starch |
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