北豆根生物碱电喷雾质谱电离规律及其特征图谱研究

用电喷雾离子阱质谱(ESI-QITMS)研究了粉防已碱和青藤碱在正离子检测方式下的一级质谱和二级质谱,总结出各自的ESI碎裂规律。依据北豆根对照药材生物碱提取物各组分的二级质谱碎裂特征进行了提取物的初步结构分析。实验发现北豆根提取物中存在曾在北豆根叶中发现的阿克吐明、阿克吐米定、阿克吐明宁3种含氯生物碱。以北豆根中16种已知主要生物碱(其中有一种存在同分异构体)为对象,应用选择离子检测(SIM)模式制作了北豆根对照药材生物碱提取物的特征指纹图谱。

Ionizing Rule and Characteristic Spectra Analysis of Electrospray Ionization for Alkaloids in Menispermum Dauricum DC.

The MS and MS2 spectra of tetrandrine and sinomenine in positive ion detection mode were analyzed by electrospray ionization quadrupole ion trap mass spectrometry (ESI-QITMS) , and their cleavage patterns were summarized. The alkaloids extracted from the medicinal materials were also analyzed using ESI-QITMS. Tetrandrine and sinomenine were identified in the extraction by comparing the MS 2 spectra of molecular ions m/z 623 and 330 with those of tetrandrine and sinomenine standards. Other known 14 ingredients were identified according to the molecular ions in MS and the characteristic product ions in MS2. Acutumine, acutumidine and acutuminine, which were three kinds of new alkaloids containing chlorine found in the leaves of Menispermun dauricum DC., were found in the extraction. The characteristic print of sixteen kinds of alkaloids (one has four kinds of isomers) in the standard medicinal materials was worked in selected ion monitor mode.