A new method for determining the local environment and orientation of individual side chains of membrane-binding peptides |
| |
Authors: | Tucker Matthew J Getahun Zelleka Nanda Vikas DeGrado William F Gai Feng |
| |
Affiliation: | Department of Chemistry, University of Pennsylvania, Philadelphia, 19104 USA. |
| |
Abstract: | We studied here the binding of the mastoparan X peptide to a zwitterionic lipid bilayer (POPC) and demonstrated that nitrile-derivatized amino acids can be used to determine the hydration state (or change in hydration state) of specific sites of membrane-interactive peptides (upon binding). We have also shown that polarized ATR-FTIR measurements can further be used to uncover information regarding the spatial orientation of individual side chains as well as their conformational preference within the lipid bilayer. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |