Untargeted screening and quantitation of drug residues in bean sprouts by ul⁃tra performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometryEI北大核心CSCD

A method for the untargeted screening and quantitative analysis of drug residues in bean sprouts by ultra performance liquid chromatography-quadrupole/ orbitrap high resolution mass spectrometry （UPLC-QOrbitrap HRMS） was developed. In the process of screening， the samples were extracted by acetonitrile and the data were gathered via the full MS-ddMS2 mode. In the absence of standards， qualitative analysis was carried out according to the exact mass number of precursor ions and secondary fragment ions information combined with the database. The optimized dispersive solid-phase extraction （d-SPE） method was used to purify the samples. 9 compounds in bean sprouts were screened and quantified on the basis of the peak areas of the precursor ions. 9 compounds showed good linear relationships （R2＞0.990） in the linear concentration ranges. The limits of detection of this method were between 2.0-5.0 μg/kg. The recoveries of 9 compounds at three spiked levels of 5， 10， 50 μg/kg ranged from 80.1% to 111.1%， and the relative standard deviations were less than 15%. © 2023, Youke Publishing Co.,Ltd. All rights reserved.