Fast enantiomeric analysis of a complex essential oil with an innovative multidimensional gas chromatographic system |
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Authors: | Mondello Luigi Casilli Alessandro Tranchida Peter Quinto Furukawa Masanao Komori Kyoichi Miseki Kozo Dugo Paola Dugo Giovanni |
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Institution: | Dipartimento Farmaco-chimico, Facoltà di Farmacia, Università degli Studi di Messina, viale Annunziata, 98168 Messina, Italy. lmondello@pharma.unime.it |
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Abstract: | The present research is focussed on the evaluation of a recently developed high performance multidimensional gas chromatographic (MDGC) system employed in the fast analysis of a series of chiral compounds contained in rosemary essential oil. The heart of the MDGC system consists in a simple transfer device for the rapid sequential re-injection of analyte "heart-cuts" from the first to the second dimension. The transfer system has no temperature restrictions, presents very low dead volumes and achieves multidimensional analysis through a pressure-balance mechanism. The MDGC set-up is characterized by two GC ovens (enabling independent temperature programming) and the possibility of mass spectrometric (MS) and/or flame ionization detection (FID). Multiple-cut conventional and fast MDGC-FID methods were developed and the results obtained compared, in order to evaluate the effectiveness of the system. In this respect, the rapid method provided the same analytical result in a greatly reduced time (approximately five times less). Furthermore, quali/quantitative data reproducibilty was very good. Fast MDGC was achieved by using micro-bore (0.1mm I.D.) columns in both dimensions. |
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