Intercalation interactions between dsDNA and acridine studied by single molecule force spectroscopy |
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| Authors: | Liu Chuanjun Jiang Zhenhua Zhang Yiheng Wang Zhiqiang Zhang Xi Feng Fude Wang Shu |
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| Institution: | Key Lab of Optoelectronics and Molecular Engineering, Department of Chemistry, Tsinghua University, Beijing, PR China. |
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| Abstract: | In this letter, we report on the direct measurement of the intercalation interactions between acridine and double-stranded DNA (dsDNA) using single molecule force spectroscopy. The interaction between acridine and dsDNA is broken by force of 36 pN at a loading rate of 5.0 nN/s. The most probable rupture force between acridine and dsDNA is dependent on the loading rate, indicating that the binding of acridine and dsDNA is a dynamic process. The combination of SMFS experimental data with the theoretical model clearly suggests the presence of two energy barriers along with an unbinding trajectory of acridine-dsDNA. |
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