Insertion of two pyrene moieties into oligodeoxyribonucleotides for the efficient detection of deletion polymorphisms |
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Authors: | Kashida Hiromu Asanuma Hiroyuki Komiyama Makoto |
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Institution: | Research Center for Advanced Science and Technology, University of Tokyo, Komaba, Meguro-ku, Tokyo, 153-8904, Japan. |
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Abstract: | For the detection of deletion polymorphisms, two pyrene moieties are tethered to an oligodeoxyribonucleotide (ODN) on both sides of the intervening base; one- and two-base deletions can be selectively detected by the strength of the excimer emission. |
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