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Convenient one-pot procedure for synthesizing 4,4′-dimethoxy-boradiaza-s-indacene dyes and their application to cell labeling |
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| Authors: | Kuan-Miao Liu Ming-Shan TsaiMing-Shiou Jan Chi-Min Chau Wan-Ju Wang |
| Institution: | a School of Applied Chemistry, Chung Shan Medical University, Taichung 40201, Taiwan, ROC b Department of Medical Research, Chung Shan Medical University Hospital, Taichung 40201, Taiwan, ROC c Institute of Microbiology and Immunology, Chung Shan Medical University, Taichung 40201, Taiwan, ROC |
| Abstract: | We succeeded in developing a convenient one-pot pathway for synthesizing 4,4′-dimethoxy-boradiaza-s-indacene dyes 4a-d. The structures feature two methoxy groups in place of the fluorine atoms in 4,4′-difluoro-4-boradiaza-s-indacene. These novel dyes emitted green fluorescence and possessed moderate to high fluorescence quantum yields (Φ=0.32-0.93). We demonstrated that these dyes have applicability to cell labeling. |
| Keywords: | 4 4&prime -Dimethoxy-boradiaza-s-indacenes Trimethyl borate BODIPY One-pot |
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