Proteomics and Detection of Uromodulin in First-time Renal Calculi Patients and Recurrent Renal Calculi Patients |
| |
Authors: | Lau Wai-Hoe Leong Wing-Seng Zhari Ismail and Gam Lay-Harn |
| |
Institution: | (1) School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 USM Penang, Malaysia;(2) Lam Wah Ee Hospital, Penang, Malaysia; |
| |
Abstract: | Renal calculi disease or known as kidney stone disease is the most common urological disorder in both men and women, although
it is more prevalent in men. The lifetime chance for an individual to develop renal calculi is ~10% whereas the risk of recurrence
in a 10-year period is 74%. Therefore, a diagnostic tool for screening or detecting renal calculi is greatly needed. In this
study, we analyze urinary protein profiles from patients with renal calculi for the first time (RC), healthy subjects (HS),
and patients with recurrent renal calculi (RRC) to identify a biomarker for detecting the disease. Urinary proteins were isolated
by salt precipitation and the proteins resolved by SDS-PAGE. Target proteins were analyzed with LC/MS/MS. Thirty-two proteins
were identified from healthy subjects and patients. Uromodulin was the most abundant urinary protein in HS but was a very
faint band if detected at all from those that formed renal calculi for the first time (p < 0.05). Yet the excreted levels of urinary uromodulin in RRC were similar to those of the HS suggesting that uromodulin
is a reliable biomarker for only RC. In addition, a few immunoglobulins that were commonly found in the urine of both RC and
RRC, which include Ig alpha heavy chain 1, Ig gamma-2 c region, Ig gamma-3 heavy chain disease protein, Ig heavy chain variable
region, Ig heavy constant region gamma 4, and Ig heavy chain. Ig heavy chain Fab frag and antibody a5b7 chain B may serve
as potential biomarkers for renal calculi disease. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|